Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent
β Scribed by Ben R. Herbert; Mark P. Molloy; Andrew A. Gooley; Bradley J. Walsh; Warren G. Bryson; Prof. Keith L. Williams
- Book ID
- 102837011
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 767 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0173-0835
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## Abstract The proteomic profiles of a human hepatoma revertant, CL1, and its original cell line, SMMC7721, were compared by using an improved twoβdimensional electrophoresis (2βDE) procedure, with multiβIPGstrips gels (length β€ 13Β cm) run simultaneously on one sodium dodecyl sulfate (SDS) gel (sh