A practicable two-dimensional electrophoresis of urinary proteins as a useful tool in medical diagnosis

✍ Scribed by DDr. Alexander Lapin; Walter Feigl

• Book ID: 102835072
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 770 KB
• Volume: 12
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150120704

✦ Synopsis

Practical experience with a rapid two-dimensional electrophoresis technique for routine analysis of urinarv proteins is discussed. The method consists of cellulose 1nstitut;Allgemeine Poliklinik der Stadt Wien acetate elecirophoresis in combination with sodium dodecyl sulfate (SDS) electrophoresis, performed together with Coomassie Blue gel staining on "PhastSystem". Over 400 analyses were performed within the time of two years. Most patients were from nephrological, urological and kidney-transplant departments. Some of them were from obstetric, pediatric or oncological departments. A systematic discussion and evaluation of the two-dimensional protein pattern with typical examples is outlined.