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Two-dimensional electrophoresis of proteins as a tool in the metabolic engineering of cell cycle regulation

✍ Scribed by Kelvin H. Lee; Michael G. Harrington; James E. Bailey

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 628 KB
Volume: 50
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(19960505)50:3<336::aid-bit12>3.0.co;2-l

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✦ Synopsis

Metabolic engineering of cell cycle regulation addresses important biotechnological problems about serum removal from animal cell culture systems. Chinese hamster ovary cells stimulated t o grow by fetal calf serum, insulin, or basic fibroblast growth factor were studied by twodimensional electrophoresis (2DE) and the resulting protein expression patterns were analyzed. Detailed 2DE protocols are provided and at least 24 gene products are identified which may play an important role in growth factor signaling. Moreover, a correlation between the expression of three proteins (cyclin D1, cyclin E, and E2F-I ) and mitogenic strength was found.

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