Improved procedures for handling thin-layer chromatographic spots

✍ Scribed by R. Amos

• Publisher: Elsevier Science
• Year: 1970
• Tongue: English
• Weight: 864 KB
• Volume: 48
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85564-3

✦ Synopsis

This paper describes improved procedures for the isolation of substances separated from complex mixtures by thin-layer chromatography for subsequent spectroscopic examination. Avoidance of contamination by plastic tubing, adsorbents, solvents and unclean glassware, as well as the problem of physical handling of small quantities of material, were all taken into account in the development of the improved procedure.

HEYNS AND GRUETZMACHER~

were among the first to combine mass spectrometry with TLC. RIX et ~1.~ recognised that sample contamination was a serious problem in TLC/MS. Without identifying specific sources, they overcame their difficulties by keeping the amounts of adsorbents, solvents and sample handling to an absolute minimum using the Pasteur-pipette capillary technique.