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Improved procedure for the preparation of DNA restriction fragments suitable for sequencing

✍ Scribed by Piergiorgio Pletta; Pierluigi Mauri; Valentina Appierto; Marina Mostardini; Ida Biunno

Book ID
112937700
Publisher
Springer-Verlag
Year
1994
Tongue
English
Weight
402 KB
Volume
44
Category
Article
ISSN
0273-2289

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In an effort to find a simple and inexpensive purification method of polymerase chain reaction (PCR) reaction before cycle sequencing reaction, we compared a commercial system with a precipitation protocol performed in our laboratory. We found that, particularly with small PCR products, our method w