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Improved method for the specific immunoprecipitation of albumin

✍ Scribed by I. D. Algranati

Publisher: Springer
Year: 1979
Tongue: English
Weight: 264 KB
Volume: 27
Category: Article
ISSN: 0300-8177
DOI: 10.1007/bf00218352

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✦ Synopsis

The immunoprecipitates of many antigens are frequently contaminated by coprecipitation of unrelated substances. A method to overcome this type of contamination in the immunoprecipitation of albumin is described. The insoluble albumin-antibody complexes are solubilized by a brief treatment at high temperature in the presence of sodium dodecyl sulfate, and after dilution the mixture is submitted to a second immunoprecipitation.

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