𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Improved method for extraction of hydroperoxide lyase fromChorella

✍ Scribed by Alberto Nuñez; Thomas A. Foglia; George J. Piazza

Book ID
104644609
Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
308 KB
Volume
9
Category
Article
ISSN
0951-208X

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✦ Synopsis

Hydroperoxide lyase converts fatty acid hydroperoxides to oxo-fatty acids which, after further oxidation, are suitable for the synthesis of higher polyamides. An improved method utilizing an acetone powder step for isolation of this enzyme from the unicellular alga Chlorella was developed. Using this procedure a five fold increase in hydroperoxide lyase activity from Chlorellu pyrenoidosu was obtained compared to previously used extraction methods. Other Chlorellu species were assayed, and it was found that C. fisca also contained significant hydroperoxide lyase activity.

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