✦ LIBER ✦

Improved method for electron microscopy immunocytochemistry of muscle

✍ Scribed by McGuffee, Linda J. ;Little, Sally A. ;Bagby, Roland M.

Publisher: Wiley (John Wiley & Sons)
Year: 1989
Tongue: English
Weight: 388 KB
Volume: 12
Category: Article
ISSN: 0741-0581
DOI: 10.1002/jemt.1060120415

No coin nor oath required. For personal study only.

✦ Synopsis

Recently, we reported a new method combining freeze fixation followed by lyophilization and embedding in Lowicryl K4M (6).

In the present brief report we compare the immunoreactivity of this preparation to chemically fixed and solvent dehydrated tissue traditionally used for electron microscopy (EM) immunocytochemistry. The primary antibody we used is a well characterized antibody to smooth muscle myosin (1'2).

📜 SIMILAR VOLUMES

Modification of grid storage boxes for p

Modification of grid storage boxes for processing in electron microscopy immunocytochemistry

✍ Cox, Robert A. ;Evans, Michael J. 📂 Article 📅 1990 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 151 KB 👁 1 views

Improved preservation of bacterila capsu

Improved preservation of bacterila capsule for electron microscopy

✍ Jacques, Mario ;Graham, Lori 📂 Article 📅 1989 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 215 KB 👁 1 views

Conventional fixation for thin-section electron microscopy is insufficient to preserve bacterial capsular material, a highly hydrated structure which collapses during dehydration and embedding. Boyles et al. (1984Boyles et al. ( , 1985) ) have recently introduced a modified aldehyde fixation process

Improved fixation ofStaphylococcus ureus

Improved fixation ofStaphylococcus ureus for electron microscopy

✍ Jones, Susan B. ;Palumbo, Samuel A. ;Smith, James L. 📂 Article 📅 1986 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 193 KB 👁 1 views

Improved uranyl acetate staining for ele

Improved uranyl acetate staining for electron microscopy

✍ Tandler, Bernard 📂 Article 📅 1990 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 154 KB

Reserve Uni versi t y , C1 eve1 and , Ohio 44106 Uranyl acetate was one o f the f i r s t contrasting agents t o be used t o s t a i n t h i n sections f o r electron microscopy (Watson, 1958). Because t h i s compound i s only sparingly soluble i n water, absolute methanol, i n which very high conc

Scanning electron microscopy of mouse mu

Scanning electron microscopy of mouse muscle microvasculature

✍ Holley, John A. ;Fahim, Mohamed A. 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 853 KB

A polylysine binding method for scanning

A polylysine binding method for scanning electron microscopy

✍ Weiss, Richard L. 📂 Article 📅 1984 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 262 KB 👁 1 views

Polylysine (PL) binding (1) has been demonstrated to be potentially useful for attaching cells and membranes to surfaces, but difficulties in reproducibility have limited its application in routine scanning electron microscopy (SEM) of single-celled