𝔖 Bobbio Scriptorium
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Improved Isolation of Anti-rhTNF-α scFvs from Phage Display Library by Bioinformatics

✍ Scribed by Wei Chen; Juan Zhang; Tao Zhang; Haixin Li; Wenyi Wang; Zhinan Xia; Min Wang


Book ID
107581718
Publisher
Humana Press Inc
Year
2009
Tongue
English
Weight
412 KB
Volume
43
Category
Article
ISSN
1073-6085

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To target CD30 on Hodgkin's disease and anaplastic large-cell lymphoma, anti-CD30 single-chain antibodies were obtained by DNA immunization of mice with the complete human CD30 cDNA. Spleens were isolated from mice with high anti-CD30 titer, and the RNA was used for the production of an scFv-display