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Isolation of ScFv antibodies of rP27kip1from phage display libraries constructed from immunized and non-immunized repertoires

โœ Scribed by Yueqiong Cao; Shouyi Qiao; Youzhong Yuan; Jiansheng Huang; Shouyuan Zhao


Book ID
105640618
Publisher
Science in China Press (SCP)
Year
1999
Tongue
English
Weight
589 KB
Volume
42
Category
Article
ISSN
1674-7305

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To target CD30 on Hodgkin's disease and anaplastic large-cell lymphoma, anti-CD30 single-chain antibodies were obtained by DNA immunization of mice with the complete human CD30 cDNA. Spleens were isolated from mice with high anti-CD30 titer, and the RNA was used for the production of an scFv-display