𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Improved isoelectric focusing of normal and variant forms of erythrocyte galactose-1-phosphate uridyl transferase

✍ Scribed by Dr. Anna M. Vaccaro; Michele Muscillo; Ida Mandara; Rosa Salvioli

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
398 KB
Volume
3
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

An improved isoelectric focusing (IEF) technique has been developed and used to detect the erythrocyte galactose‐1‐phosphate uridyl transferase isoenzymes. The normal enzyme was resolved by IEF in four bands with isoelectric points (pI) between 5.80 and 6.08. Numerous samples, classified according to electrophoresis as Duarte or Los Angeles homozygotes, showed the same IEF pattern composed of four bands with pI's between 5.60 and 5.90. The Duarte‐normal and Los Angelesnormal heterozygotes gave an IEF pattern of 4‐5 bands (pI 5.60‐6.08), with relative intensities that varied according to their activity.

📜 SIMILAR VOLUMES

Enzyme kinetics in mammalian cells. III.
Enzyme kinetics in mammalian cells. III. Regulation of activities of galactokinase, galactose-1-phosphate uridyl transferase and uridine diphosphogalactose-4-epimerase in human erythrocytes
✍ Helene Z. Hill 📂 Article 📅 1971 🏛 John Wiley and Sons 🌐 English ⚖ 874 KB

The sequential enzyme assay as previously described has been used to study various effects on the three enzymes in human red cells involved in the phosphorylation of galactose : galactokinase, galactose-1-phosphate uridyl transferase and uridine diphospho-galactose-4-epimerase. 1. Enzyme activities