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Improved indirubin production in a two-phase suspension culture of Polygonum tinctorium using dimethylpolysiloxane

โœ Scribed by G.W. Bae; C.S. Chung; K.I. Kim; C.H. Park; H.J. Lee; Y.A. Chae; I.S. Chung

Book ID
110267837
Publisher
Springer-Verlag
Year
1998
Tongue
English
Weight
129 KB
Volume
12
Category
Article
ISSN
0951-208X

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Stimulation of indirubin production by KNO3depletion in indole-supplemented suspension culture ofPolygonum tinctorium
โœ I. S. Chung; T. Kim; G. W. Bae; H. J. Lee; Y. A. Chae ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 241 KB

Indole when added at 20 mM to shake flask cultures of Polygonum tinctorium cells on 16th day increased indirubin production from 41 mg/l to 126 mg/l. Use of KNO3 depleted media stimulated indirubin formation up to 88% and 26% in shake flasks and air-lift bioreactors, respectively.