Improved in vitro gene transfer mediated by fluorinated lipoplexes in the presence of a bile salt surfactant

✍ Scribed by Jérôme Gaucheron; Catherine Santaella; Pierre Vierling

• Book ID: 102341772
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 199 KB
• Volume: 3
• Category: Article
• ISSN: 1099-498X
• DOI: 10.1002/jgm.188

✦ Synopsis

Background Progress in the field of gene transfer with non-viral vectors requires systems that allow efficient gene expression in the presence of biological fluids such as pulmonary surfactants, for gene transfer to the respiratory epithelium for cystic fibrosis gene therapy, or bile salts (which contain powerful anionic detergents), for gene transfer to the biliary epithelium for gene therapy of the hepatobiliary disease associated with cystic fibrosis (CF). We have performed a comparative analysis of the disintegration and DNA accessibility of fluorinated and conventional lipoplexes, and their in vitro transfection potential in the presence of a powerful biliary surfactant.