Improved extraction procedure for the isolation of β-D-galactosidase from Escherichia coli
✍ Scribed by Annamária Szöke; Rocco Campagna; Karl-Heinz Kroner; Helmut Hustedt
- Publisher
- Springer-Verlag
- Year
- 1988
- Tongue
- English
- Weight
- 283 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0951-208X
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📜 SIMILAR VOLUMES
The enzyme-catalyzed hydration2 of D-galactal and the enzyme-catalyzed hydrolysis of fl-D-galactopyranosides are similar as far as the products are concerned. In the case of hydration of D-galactal, a covalent, 2deoxy-D-Zyxo-hexosy1("2deoxy-D-galactosyl")enzyme intermediate could be isolated' . As s
beta-D-Galactosidase, isolated from cloned E. coli, was immobilised on cellulose beads via oxidation with sodium periodate, activation by cyanuric chloride, or diazotisation. beta-D-Galactosidase immobilised via azo bonds showed the highest relative activity and thermostability, and was used for syn