Improved Extraction of Abscisic Acid From Plant Tissue
โ Scribed by Loveys, BR ;van Dijk, HM
- Book ID
- 120808347
- Publisher
- Commonwealth Scientific and Industrial Research Organisation Publishing
- Year
- 1988
- Tongue
- English
- Weight
- 351 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1445-4408
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A method for reproducibly estimating the protoheme content of plant tissues has been developed. The tissue sample is homogenized in 80% acetone to remove pigments and lipids; protoheme is then extracted from the tissue residue with 2% HCl in acetone and quantitatively transferred into diethyl ether.
Methods are given for the quantitation of the plant stress hormone, abscisic acid (ABA), and its two metabolic precursors, ketone and enolate, that are applicable to all species tested so far. The plant extract is homogenized at neutral pH, hexane-soluble neutrals are extracted and discarded, and th