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Improved electrophoretic methods for identifying cereal varieties

✍ Scribed by Diana L. Du Cros; Colin W. Wrigley

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
653 KB
Volume
30
Category
Article
ISSN
0022-5142

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✦ Synopsis

Abstract

Several electrophorectic methods are compared in terms of their reliability, convenience and ability to distinguish between varieties of wheat, barley, rye and triticale using proteins extracted from the endosperm with 1M urea. Gradient gel electrophoresis offers an improvement, particularly in convenience, over the use of starch gels and uniform‐pore polyacrylamide. Varietal identification is described using gels having gradients in polyacrylamide concentration of 3‐27% or 2‐16%, both being available commercially ready for use. Electrophoresis in the latter gels at 400 V takes only half an hour for barley and an hour for wheat. In addition, a simple procedure is described for gel isoelectric focusing which, used alone or in combination with gel electrophoresis, provides high resolution of endosperm proteins for distinguishing between closely related varieties.

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