Eingegangen am 2 . 9.1983) Xystatin was used to develop a new method to selert spores of the yeast Saccharomycopsis lipolytica. At low concentrations nystatin killed preferently growing cells of this yeast. At high concentrations nongrowing cells were affected as well. I n contrast, spores mere not
Improved conditions for mating of the yeast Saccharomycopsis lipolytica
โ Scribed by G. Barth; H. Weber
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 231 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0233-111X
No coin nor oath required. For personal study only.
โฆ Synopsis
Conditions for mat,ing of Sacchnromycopsis lipolyfica cells were investigated to cnhance zygote format,ion. Conjugat,ion frequencies could be increased about twenty five fold by rising ce11s of tho late logarithmic growt)h phase and a yeast'-extract-malt medium t'o induce ma'ting. A procedure based o n thcsr: results is descrihcd and has been used to carry out complrmentat.ion anitlysis in 8. Zipolyticn much more rapidly.
๐ SIMILAR VOLUMES
## Development of breeding stocks of the yeast Saccharomycopsis lipolytica by methods of moderate inbreeding CORNELIA KURISCHKO, S. G. INGE-VECHTOMOV~) and H. WEBER (Eingegangen am 8. 2. 1983) Sporulation parameters of genetically labelled strains, derived from a wild strain of the alkaneutilizing
Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-f