Improved assay of coenzyme F420analogues from methanogenic bacteria
β Scribed by Michael W. Peck; David B. Archer
- Publisher
- Springer-Verlag
- Year
- 1987
- Tongue
- English
- Weight
- 283 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0951-208X
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β¦ Synopsis
An improved method for separating analogues of coenzyme F420 by isocratic reversed-phase high performance liquid chromatography is described. The method offers improved resolution, shorter chromatography runs and requires less complex apparatus.
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## ~ ~~ F430M, the pentamethyl ester of coenzyme F430, can be oxidized reversibly by one electron. The oxidation potential has been determined, and the electrolytically prepared oxidation product was characterized by its UVjVIS and ESR spectrum. The strongly anisotropic and nearly axial ESR spectr
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