Improved antigen detection on western blots
โ Scribed by Hiroshi Matsugu; Takahito Nishikata
- Book ID
- 114393124
- Publisher
- Elsevier
- Year
- 2001
- Weight
- 159 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1366-2120
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## Detection of lectin activity on Western blots using erythrocytes A method is described for the direct detection of lectins, agglutinating erythrocytes, on nitrocellulose membranes after Western blotting, thus avoiding protein extraction from specific bands in the gel, followed by agglutination
A reliable sequential application of peroxidase and alkaline phosphatase-conjugated second antibodies to detection of two different antigens in Western blots is described. Deposits of differently colored products formed allow identification of several antigens on a single blot.