Improved Allele-Specific PCR Technique for Kidd Blood Group Genotyping
β Scribed by Intharanut, Kamphon; Grams, Rudi; Bejrachandra, Sasitorn; Sriwanitchrak, Pramote; Nathalang, Oytip
- Book ID
- 118762641
- Publisher
- John Wiley and Sons
- Year
- 2013
- Tongue
- English
- Weight
- 535 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0887-8013
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An allele-specific polymerase chain reaction (ASPCR) assay for prenatal genotyping of the Kidd antigen system in order to identify pregnancies at risk for haemolytic disease of the newborn (HDN) was developed. Oligonucleotide primers were designed for ASPCR of JKA and JKB. A validation study was per
## Communicated by Henrik Dahl PCR permits direct genotyping of individuals at the ABO locus. Several methods have been reported for genotyping ABO that rely on differentiating the A, B, and 0 alleles at specific base substitutions. However, the 0 allele as defined by serology comprises at least t