A rapid and reliable PCR method for genotyping the ABO blood group. II: A2 and O2 alleles

✍ Scribed by Denise S. O'Keefe; Alexander Dobrovic

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 470 KB
• Volume: 8
• Category: Article
• ISSN: 1059-7794
• DOI: 10.1002/(sici)1098-1004(1996)8:4<358::aid-humu9>3.0.co;2-3

✦ Synopsis

Communicated by Henrik Dahl

PCR permits direct genotyping of individuals at the ABO locus. Several methods have been reported for genotyping ABO that rely on differentiating the A, B, and 0 alleles at specific base substitutions. However, the 0 allele as defined by serology comprises at least two alleles (0' and 0') at the molecular level, and most current ABO genotyping methods only take into account the 0' allele. Determining the presence of the 0' allele is critical, as this not-infrequent allele would be mistyped as an A or a B allele by standard PCR typing methods. Furthermore, none of the methods to date distinguish between the A' and A' alleles, even though 10% of all white persons are blood group A'. We have developed a method for genotyping the ABO locus that takes the 0' and A' alleles into account. Typing for A' and 0' by diagnostic restriction enzyme digestion is a sensitive, nonradioactive assay that provides a convenient method useful for forensic and paternity testing and for clarifying anomalous serological results.