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Import of the precursor of the chloroplast Rieske iron-sulphur protein by pea chloroplasts

✍ Scribed by A. Hugh Salter; Barbara J. Newman; Johnathan A. Napier; John C. Gray

Publisher: Springer
Year: 1992
Tongue: English
Weight: 557 KB
Volume: 20
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00040617

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✦ Synopsis

cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino acid residues, of which 50 residues constitute an N-terminal cleavable presequence and 180 residues make up the mature protein. Transcription and translation of the cDNA in vitro produced a polypeptide of 26 kDa which was efficiently imported by isolated pea chloroplasts and processed to the mature 20 kDa protein. Southern hybridisation to pea genomic DNA indicated the presence of a single gene encoding the Rieske FeS protein in the haploid genome.

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