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Import and processing of the precursor of the delta subunit of tobacco chloroplast ATP synthase

✍ Scribed by J. A. Napier; K. H. Larsson; F. Madueño; J. C. Gray

Publisher
Springer
Year
1992
Tongue
English
Weight
527 KB
Volume
20
Category
Article
ISSN
0167-4412

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✦ Synopsis

A cDNA clone encoding the complete precursor of the b subunit of chloroplast ATP synthase has been isolated from a tobacco (Nicotiana tabacum) leaf cDNA library in 2gt11. The 880 bp insert encodes a polypeptide of 248 amino acid residues, of which 61 residues constitute an N-terminal presequence and 187 residues make up the mature b subunit. Transcription and translation of the cDNA in vitro produced a protein of 29 kDa which was imported by isolated pea chloroplasts and processed to the mature 20 kDa subunit. The b subunit precursor was processed to the mature size by a processing peptidase present in pea stromal extracts. Hybridisation of the cDNA to Southern blots of tobacco genomic DNA suggests the presence of two genes in the haploid genome.

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