Implementation of the chemical reaction interface mass spectrometry technique on a Hewlett-Packard mass-selective detector

✍ Scribed by Hengchang Song; Jozef Kusmierz; Fred Abramson; Matt McLean

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 687 KB
• Volume: 5
• Category: Article
• ISSN: 1044-0305
• DOI: 10.1016/1044-0305(94)80009-x

✦ Synopsis

A microwave-powered chemical reaction interface has been installed in a Hewlett-Packard gas chromatograph-mass spectrometer (GC-MS) system (5890 IT gas chromatograph-S971 mass-selective detector). The technical details and optimization strategies are discussed. The evaluation of this new setup is presented, showing detection limits of 1 ng of (13)C-, (15)N-, and Cl-containing compounds with signal-to-noise ratios greater than or egual to 3. Selective detection was evaluated with a urine sample from a dog dosed with (15)N3-midazolam that had been previously analyzed by using a differentially pumped research-level quadrupole mass spectrometer. The results show that the detection of (15)N and Cl remains highly selective and the mass-selective detector gives comparable sensitivity to the larger instrument when the latter is operating over a conventional mass range. The capability for chemical reaction interface mass spectrometry can be easily accomplished with an inexpensive GC-MS system.