Imperfect complementation in human-hamster somatic cell hybrids
β Scribed by TALAVERA, ANTONIO; BASILICO, CLAUDIO; CROCE, CARLO M.
- Book ID
- 109698365
- Publisher
- Nature Publishing Group
- Year
- 1976
- Tongue
- English
- Weight
- 467 KB
- Volume
- 259
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/259667a0
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π SIMILAR VOLUMES
Lymphocy tes from a patient with classic galactosemia (GA LT deficiency) were hybridized with a Chinese hamster cell line. Electrophoretic evaluation of GAL T in 31 independently derived interspecific hybrid clones failed to demonstrate expression of the human GALT gene even when human chromosome 9
About 75 man-Chinese hamster hybrid clones were analysed for their human chromosome complement and simultaneously tested for human enzyme markers. Correlation of the presence of chromosomes and enzyme activity revealed assignments of the PGD linkage group to chromosome 1, ME1, PGM a and IPO-B to 6,