Impedimetric Detection of Hairpin Ribozyme Activity

✍ Scribed by Katarzyna Piekielska; Magdalena Gębala; Sławomir Gwiazda; Sabine Müller; Wolfgang Schuhmann

• Book ID: 102184403
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 341 KB
• Volume: 23
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.201000640

✦ Synopsis

Abstract

Electrochemical impedance spectroscopy is successfully utilized for label‐free monitoring of the cleavage reaction of a RNA substrate by a complementary hairpin ribozyme. The formation of the RNA substrate/ribozyme complex is increasing the negative charge at the interface and is modulating the kinetic of the redox conversion of a negatively charged redox mediator (e.g. [Fe(CN)~6~]^3−/4−^) hence leading to an increase in the charge transfer resistance. Upon addition of bivalent cations such as Mg^2+^ ions the conformation of the ribozyme is changed and the catalytic cleavage of the RNA is monitored by a decrease of the charge transfer resistance.