Clonogenic cells from peripheral blood of I3/ I6 patients with T-cell malignancies generated colonies in methykellulose in absence of added growth factors or mitogenic stimulation. Spontaneous colonies were also obtained from purified cell fractions (E-OKT3-and/or E+ cells) in 13 7 ' and 57 % of the
Impaired T-lymphocyte colony formation by cord blood mononuclear cells
β Scribed by Henry G. Herrod; William R. Valenski
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 978 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0271-9142
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Mononuclear cells isolated from human cord blood (CBL) of full-term neonates were stimulated in vitro with a dose range of T cell-dependent antigens, i.e. ovalbumin or sheep erythrocytes, and tested for the capacity to mount an antigen-specific plaque-forming cell (PFC) response. Both of the antigen
## Abstract ## Background Immunophenotyping of whole blood (WB) and isolated peripheral blood mononuclear cells (PBMCs) is a common tool used to evaluate immune system changes in clinical studies. The development of methods that would allow preservation of samples for flow cytometric analysis is i