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Colony formation in the absence of added growth factors by peripheral blood T-cell colony-forming cells of patients with T-cell malignancies

✍ Scribed by Vassilis Georgoulias; Aldar Bourinbaiar; Françoise Amesland; Chantal Canon; Huguette Auclair; Claude Jasmin

Publisher
John Wiley and Sons
Year
1984
Tongue
French
Weight
730 KB
Volume
34
Category
Article
ISSN
0020-7136

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✦ Synopsis

Clonogenic cells from peripheral blood of I3/ I6 patients with T-cell malignancies generated colonies in methykellulose in absence of added growth factors or mitogenic stimulation. Spontaneous colonies were also obtained from purified cell fractions (E-OKT3-and/or E+ cells) in 13 7 ' and 57 % of the patients, respectively. No spontaneous colony growth was observed with mononuclear cells of patients with solid tumors, non-T-cell leukemias or normal subjects. Colonies consisted of acid-phosphatase-positive, myeloperoxidase-and PAS-negative lymphoblasb bearing T-cell surface markers. Although the phenotype of pooled colony cells from either unfractionated mononuclear cells or E-OKT3-4erived colonies varied from patient to patient, the colonies, like fresh leukemic cells, were mostly composed of relatively immature cells as assessed by the high proportion (>40%) of OKT6+ and OKTIO+ cells and the low proportion (<a%) of OKT3+ and/or E+ cells. Cytogenetic analysis of colony cells revealed either normal metaphases or chromosome anomalies similar to those observed in fresh leukemic cells. Moreover, cells from primary colonies exhibited a capacity for self-renewal in the absence of added growth factors.

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