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Impact of Milk Components on Recovery of Viral RNA from MS2 Bacteriophage

โœ Scribed by Yavarmanesh, M.; Abbaszadegan, M.; Alum, A.; Mortazavi, A.; Habibi Najafi, M. B.; Bassami, M. R.; Nassiri, M. R.

Book ID
119996403
Publisher
Springer-Verlag
Year
2013
Tongue
English
Weight
327 KB
Volume
5
Category
Article
ISSN
1867-0334

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## Abstract MS2 RNA, which sediments at 27S in a neutral buffer, can be converted to a compact 57S conformation at pH 3.8. Requirements for this conversion, besides protonation, are small concentrations of Mg^++^ ions and a low ionic strength. On the other hand, after heating in the presence of EDT