Impact of Lipid Substitution on Assembly and Delivery of siRNA by Cationic Polymers

✍ Scribed by Hamidreza Montazeri Aliabadi; Breanne Landry; Remant K. Bahadur; Artphop Neamnark; Orawan Suwantong; Hasan Uludağ

• Book ID: 102934874
• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 875 KB
• Volume: 11
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.201000402

✦ Synopsis

Abstract

Characterization of a polymer library engineered to enhance their ability to protect and deliver their nucleotide cargo to the cells is reported. The ζ‐potential continuously increased with higher polymer:siRNA weight ratio, and the ζ‐potential of lipid‐modified polymers:siRNA complexes were higher than PEI2 at all ratios. At polymer:siRNA ratio of 1:1, all lipid‐substituted polymers showed complete protection against degradation. Lipid‐modified polymers significantly increased the cellular uptake of siRNA complexes and down‐regulation of GAPDH and P‐gp (max. 66% and 67%, respectively). The results indicate that hydrophobic modification of low molecular PEI could render this otherwise ineffective polymer to a safe effective delivery system for intracellular siRNA delivery and protein silencing.
magnified image