A system for detecting a spontaneous deletion in Escherichia coli was developed and the role of DNA gyrase in deletion formation was studied. A derivative of 2plac5, 2AM36, was isolated in which whole pBR322 DNA was inserted in the lacZ gene and 227 bp of the lac gene duplicated at both sides of the
β¦ LIBER β¦
Impact of DNA gyrase inhibition by antisense ribozymes on rec A inE. coli
β Scribed by Sainath Rao Shilpakala; Malathi Raghunathan
- Publisher
- Springer
- Year
- 2008
- Tongue
- English
- Weight
- 251 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0301-4851
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In the absence of nucleotide excision repair, the spectra were dominated by G:CrA:T transitions, additional deficiency of the DNA alkyltransferase consistent with the major role of the O 6 -alkylguanine (ATase) encoded by the constitutive ogt gene of miscoding lesion in mutagenesis by alkylating Esc