Immunoprecipitation of phytochrome from greenAvenaby rabbit antisera to phytochrome from etiolatedAvena

✍ Scribed by Y. Shimazaki; L. H. Pratt

• Publisher: Springer-Verlag
• Year: 1986
• Tongue: English
• Weight: 330 KB
• Volume: 168
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00392270

✦ Synopsis

Thirty-nine antiserum preparations from eight rabbits were screened for their ability to precipitate the immunochemically distinct phytochrome that is obtained from green oat (Avena sativa L.) shoots. The antisera were obtained from rabbits immunized with either proteolytically degraded, but still photoreversible, 60-kDa (kilodalton) phytochrome, or approx. 120-kDa phytochrome, both of which were purified from etiolated oat shoots. The ability of these antisera to precipitate phytochrome from green oats was independent of the size of phytochrome used for immunization. While crude antisera immunoprecipitated as much as 80% of the phytochrome isolated from green oat shoots, antibodies immunopurified from these sera with a column of highly purified, approx. 120-kDa phytochrome from etiolated oats precipitated no more than about 5-10%.