Immunological studies on mouse mammary tumors. I. Induction of resistance to tumor isograft in C3H/He mice

✍ Scribed by Kusuya Nishioka; Reiko Furuse Irie; Masaharu Inoue; Shaoyuan Chang; Shoshichi Takeuchi

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: French
• Weight: 621 KB
• Volume: 4
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910040202

✦ Synopsis

An isografted tumor MM2, originating from a spontaneous mammary tumor in a CjlHIHelrnouse, killed 4-to 5-week-old mice within 30 days through intraperitoneal injection of 2 x lo4 cells per mouse. It was shown that resistance to the isografi was induced by injection of 1.5 x lo5 or 2.0 x lo5 tumor cells sensitized with serum ( 5 pg antibody N ) of rabbits immunized with the tumor, Four weeks after injection of the sensitized M M 2 cells, I73 C3HIHe mice were challenged intraperitoneally with fresh unsensitized M M 2 in doses of 5 x lo5 to 1 x lo6 cells per mouse. Of these, 119 mice survived without any sign of tumor growth while all untreated mice died. The mean survival time for untreated mice was 18.1 days ( 5 1 . 7 ) when inoculated with 5 x lo5 cells and 16.2 days (&1.8) when inoculated with 1 ~1 0 ' cells. After the second challenge with the same number of fresh unsensitized cells, 117 out of 119 mice survived without any sign of tumor growth. All of 15 mice randomly selected from these resistant mice were tested for acceptance of skin grafis from normal C3HIHe mice. The grafis showed no rejection even IS0 days after the second graft. After hyperimmunization of these resistant mice, the serum of the spleen extract taken from the mice inhibited growth of tumors when the tumors were premixed in vitro and injected intraperitoneally into C3HIffe mice,