Immunological identification of two lamina-like proteins inSaccharomyces cerevisiae
โ Scribed by Zoya Galcheva-Gargova; Lubomira Stateva
- Book ID
- 112482628
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 333 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0144-8463
No coin nor oath required. For personal study only.
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## Background Sortin2 is a low mass compound that interferes with vacuolar delivery of proteins in plants and yeast. The Sortin2 phenotype was tested in __Arabidopsis thaliana__ and found to be reversible upon drug removal, demonstrating the ability of chemical genomics to induce reversible phenoty
The two-hybrid system was used to identify yeast genes encoding proteins that interact with DNA topoisomerase I. Two new genes were found and named TOF1 and TOF2. The Tof1 protein has 1238 amino acids and no obvious homologues in databases. The Tof2 protein has 771 amino acids; it is quite closely r