Intact Saccharomyces cerevisiae cells were biotinylated with the non-permeable sulfosuccinimidyl-6-(biotinamido)hexanoate reagent. Twenty specifically labelled cell wall proteins could be extracted and visualized on SDS gels via streptavidin/horseradish peroxidase. Nine cell wall proteins were relea
Sequence-based approach for identification of cell wall proteins inSaccharomyces cerevisiae
β Scribed by Hiromichi Terashima; Satoshi Fukuchi; Kenta Nakai; Mikio Arisawa; Kenji Hamada; Nami Yabuki; Kunio Kitada
- Publisher
- Springer-Verlag
- Year
- 2002
- Tongue
- English
- Weight
- 269 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0172-8083
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Use of the Von Heijne algorithm allowed the identification of 686 open reading frames (ORFs) in the genome of Saccharomyces cerevisiae that encode proteins with a potential N-terminal signal sequence for entering the secretory pathway. On further analysis, 51 of these proteins contain a potential gl
In fungi, cell shape is determined by the presence of a rigid cell wall which separates the cell from the extracellular medium. This highly dynamic structure is essential for the maintenance of cell integrity and is involved in several phenomena such as flocculation, adherence and pathogenicity. The
Protoplasts of Saccharomyces cerevisiae incubated in regenerating conditions secrete cell wall components in order to allow the biosynthesis of this structure. During the first hours of incubation, many of these are not retained in the forming cell wall but remain in the medium. We have developed a