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Immunological characterization of the RNA-dependent DNA polymerase from reticuloendotheliosis virus

โœ Scribed by Karin Moelling


Publisher
Springer-Verlag
Year
1977
Tongue
English
Weight
201 KB
Volume
164
Category
Article
ISSN
0300-8584

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A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, a