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Immunolocalization of phosphoprotein B23 in proliferating and non-proliferating hela cells

✍ Scribed by Benjamin Y-M. Yung; Amy M-s. Bor; Y. H. Yang

Publisher: John Wiley and Sons
Year: 1990
Tongue: French
Weight: 713 KB
Volume: 46
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910460222

No coin nor oath required. For personal study only.

✦ Synopsis

Localization of protein 823 in HeLa cells under different growth conditions was studied using indirect immunofluorescence. Bright nucleolar fluorescence was observed in exponentially growing cells. After 3 to 4 days, the initial cell inocula entered a phase of stationary growth as defined by no increments in cell number. The nucleolar fluorescence was then diminished and a general nuclear immunofluorexence was observed. This chan e in localization of fluorescence indicated that protein B2f had migrated out of the nucleoli during the suboptimal growth conditions. Relocation of protein 823 in nucleoli was observed in cells of stationary growth after treatment with adriamycin or daunomycin at their subtoxic concentrations ( M). Adriamycin and daunomycin, at their toxic concentrations ( S . 0 X lo-' M), on the other hand, inhibited cell growth and induced B23 translocation from nucleoli to nucleoplasm in growing cells. These results indicate that both adriamycin and daunomycin exhibit biphasic effects on the proliferation of cells by either stimulation or inhibition depending on the drug concentrations and the growth conditions. B23 translocation, as observed by immunofluorescence may be a simple and rapid method for assessing inhibition-stimulation growth response to anti-tumor therapy.

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