𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Glutamylation of centriole and cytoplasmic tubulin in proliferating non-neuronal cells

✍ Scribed by Bobinnec, Y. ;Moudjou, M. ;Fouquet, J.P. ;Desbruyères, E. ;Eddé, B. ;Bornens, M.

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
253 KB
Volume
39
Category
Article
ISSN
0886-1544

No coin nor oath required. For personal study only.

✦ Synopsis

We have examined the distribution of glutamylated tubulin in non-neuronal cell lines. A major part of centriole tubulin is highly modified on both the ␣and ␤-tubulin subunits, whereas a minor part of the cytoplasmic tubulin is slightly modified, on the ␤-tubulin only. Furthermore, we observed that tubulin glutamylation varies during the cell cycle: an increase occurs during mitosis on both centriole and spindle microtubules. In the spindle, this increase appears more obvious on the pole-to-pole and kinetochore microtubules than on the astral microtubules. The cellular pattern and the temporal variation of this post-translational modification contrast with other previously described tubulin modifications. The functional significance of this distribution is discussed.

📜 SIMILAR VOLUMES

Immunolocalization of phosphoprotein B23
Immunolocalization of phosphoprotein B23 in proliferating and non-proliferating hela cells
✍ Benjamin Y-M. Yung; Amy M-s. Bor; Y. H. Yang 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 French ⚖ 713 KB

Localization of protein 823 in HeLa cells under different growth conditions was studied using indirect immunofluorescence. Bright nucleolar fluorescence was observed in exponentially growing cells. After 3 to 4 days, the initial cell inocula entered a phase of stationary growth as defined by no incr

Differential localization of acetylcholi
Differential localization of acetylcholinesterase in neuronal and non-neuronal cells
✍ Matthew D. Thullbery; Holly D. Cox; Travis Schule; Charles M. Thompson; Kathleen 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 291 KB

## Abstract Acetylcholinesterase (AChE) expression is regulated in cell types at the transcriptional and translational levels. In this study, we characterized and compared AChE catalytic activity, mRNA, protein expression, and protein localization in a variety of neuronal (SH‐SY5Y neuroblastoma and

Postnatal proliferation of DRG non-neuro
Postnatal proliferation of DRG non-neuronal cells in vitamin E-deficient rats
✍ Cecchini, Tiziana ;Ferri, Paola ;Ciaroni, Sandra ;Cuppini, Riccardo ;Ambrogini, 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 235 KB

Changes in the number of satellite cells in neuron body sheaths in dorsal root ganglia (DRGs) were studied from 1 to 5 months of age in control and in vitamin E-deficient rats; furthermore, the satellite cell proliferation rate was detected in the same groups of animals with immunohistochemistry for

Study on the toxic mechanism of prion pr
Study on the toxic mechanism of prion protein peptide 106–126 in neuronal and non neuronal cells
✍ Ingrid Dupiereux; Willy Zorzi; Walid Rachidi; Danièle Zorzi; Olivier Pierard; Be 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 591 KB

## Abstract A synthetic peptide corresponding to the 106–126 amyloidogenic region of the cellular human prion protein (PrP^c^) is useful for in vitro study of prion‐induced neuronal cell death. The aim of the present work was to examine the implication of the cellular prion protein in the toxicity