Immunohistochemical localization of TGFβ1, TGFβ2, and TGFβ3 in normal and malignant human prostate

✍ Scribed by Perry, Kent T.; Anthony, Catherine T.; Steiner, Mitchell S.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 577 KB
• Volume: 33
• Category: Article
• ISSN: 0270-4137
• DOI: 10.1002/(sici)1097-0045(19971001)33:2<133::aid-pros7>3.0.co;2-l

✦ Synopsis

Background:

Prostate cancer eventually becomes androgen-independent, suggesting that growth factors such as tgf beta 1-3 may potentially contribute to prostate neoplasia. the pattern and level of tgf beta 1-3 protein expression in normal and malignant human prostate are unknown.

Methods:

An immunohistochemical study was undertaken to analyze tgf beta 1, tgf beta 2, and tgf beta 3 protein in malignant and adjacent normal prostates from 25 patients who had clinically localized prostate cancer.

Results:

Normal prostate exhibited similar tgf beta 1 immunostaining in stromal and epithelial cells, whereas tgf beta 2 and tgf beta 3 protein staining was greater in the epithelial relative to the stromal compartments. in malignancy, prostate epithelial cells had higher tgf beta 1 and tgf beta 2 immunostaining than either the surrounding stromal cells or their normal prostatic epithelial counterparts. although tgf beta 3 staining intensity was similar for both malignant and normal prostate epithelial cells, the pattern of staining switched from uniform apical to diffuse protein staining in malignant prostate glands.

Conclusions:

Prostate cancer was associated with alterations of tgf beta 1, tgf beta 2, and tgf beta 3 expression by prostatic epithelial cells which may play a role in prostatic carcinogenesis.