Immunohistochemical differential distribution of S-100α and S-100β in the peripheral nervous system of the rat

✍ Scribed by Dr. Kimiya Sugimura; Dr. Hajime Haimoto; Dr. Hiroshi Nagura; Dr. Kanefusa Kato; Akira Takahashi

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 640 KB
• Volume: 12
• Category: Article
• ISSN: 0148-639X
• DOI: 10.1002/mus.880121109

✦ Synopsis

The localization of the a subunit of the S-100 protein (S-100a) and p subunit (5-loop) was studied in the peripheral nervous system of the rat. In peripheral nerves, S-100a and S-1OOp were found in the cytoplasm of Schwann cells. Axons were positively stained in part by S-1 0 0 ~ and almost totally by S-loop. In the dorsal root ganglia, S-1OOa was found in satellite cells and their processes and in some neurons. S-1 OOp was found in more of the large neurons, but almost all of the small neurons were negative for S-I OOp. In the anterior horn cells, S-1 OOp staining was stronger than that of S-100a. In Schwann cells, both S-100a and S-loop were present on the rough endoplasrnic reticulum, free ribosomes, and nucleus, as seen by electron microscopy. The S-1OOa and S-loop in axons were associated with rnicrotubules and neurofilarnents. Key words: immunohistochemistry electron microscopic immunohistochemistry S-1OOa S-loop peripheral nervous system rat Schwann cell axon satellite cell neuron MUSCLE & NERVE 12:929-935 1989