Different distributions of immunoreactive S100-α and S100-β protein expression in human breast cancer

✍ Scribed by Funahashi, Hiroomi; Koshikawa, Takashi; Ichihara, Shu; Ohike, Ekoh; Katoh, Kanehusa

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 625 KB
• Volume: 68
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/(sici)1096-9098(199805)68:1<25::aid-jso6>3.0.co;2-n

✦ Synopsis

Background and Objectives: Although the localization of S100 protein in breast carcinoma has previously been studied, the immunohistochemical expression of the S100-␣ and -␤ subunits has not been examined. Methods: Immunohistochemical staining for S100-␣ and S100-␤ proteins was performed on 72 benign breast lesions and 72 infiltrating ductal carcinoma of the breast. Noncross-reactive anti-S100-␣ and anti-S100-␤ antibodies purified by affinity chromatography were used in the studies. Results: More than 30% of the epithelial cells comprising all the benign lesions were either S100-␣ or S100-␤ positive. In breast carcinoma cases, however, >30% of malignant cells were S100-␣ positive in 70/72 cases (97.2%), whereas the number of S100-␤ positive cells exceeded 30% in only 3/72 cases (4.0%). Conclusions: Immunohistochemical staining for S100-␣ and S100-␤ proteins may help to differentiate benign proliferative breast lesions from breast cancers in difficult cases.