Immunochemical studies of organ and tumor lipids. IV. Chromatographic behavior of the lipid hapten of rat lymphosarcoma: Dependence of in vitro measurements on lipid interactions

✍ Scribed by Maurice M. Rapport; Nicholas F. Alonzo; Liselotte Graf; Vladimir P. Skipski

• Publisher: John Wiley and Sons
• Year: 1958
• Tongue: English
• Weight: 824 KB
• Volume: 11
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(195811/12)11:6<1125::aid-cncr2820110607>3.0.co;2-s

✦ Synopsis

T IS A WELL established phenomenon that I the antibodies produced in rabbits by the injection of certain mammalian tissue fractions may react with alcoholic extracts of the tissue. The nature of the reactive substances in the alcoholic extracts is not known, but by virtue of their similarity in extractability and incomplete antigenicity to the Wassermann hapten (cardiolipin) and the Forssman hapten they are called lipid or "lipid soluble" haptens.

There are several cogent reasons for studying these substances. In the first place, a considerable degree of both species and organ specificity is associated with them, and therefore they may prove useful in establishing cellular relationships. In the second place, they may be measured quantitatively in exceedingly small amounts, and therefore they are potentially useful for diagnostic purposes. And finally, if they should be of low molecular weight, they might provide much information on the relation of chemical structure to antigenicity for an important class of cellular components.

For the past 20 years, this field has not attracted much attention-partly because of certain inadequacies in attempting to interpret the data and to transmit the information acquired by measurements based on complement fixation, and partly as a result of the lack