Immuno-polymerase chain reaction for detection ofAspergillus fumigatus
β Scribed by P. Usha Sarma; Nivedita Bir; Anubha Paliwal; Prasad Reddy
- Publisher
- Association of Clinical Biochemistry of India
- Year
- 1997
- Tongue
- English
- Weight
- 505 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0970-1915
No coin nor oath required. For personal study only.
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All articles available online at http://www.idealibrary.com on FIG. 3. Correlation of VEGF concentrations in 40 normal human serum samples measured by fluorometric ELISA ( y axis) and immuno-PCR ( x axis) ( y Ο 0.76 x Οͺ 7 pg/ml, correlation coefficient Ο 0.83, P Ο½ 0.0001).
A powerful method to amplify reverse-transcribed RNA, the polymerase chain reaction can be used to measure cytokine gene transcription in a small number of cells, or in cases where there is low mRNA copy number. This technique may be used to obtain qualitative or quantitative determinations of cytok