Immune response and gene polymorphism profiles in Crohn's disease and ulcerative colitis

✍ Scribed by Dulciene Maria Magalhães Queiroz; Adriana Gonçalves Oliveira; Ivan Euclides Borges Saraiva; Gifone Aguiar Rocha; Andreia Maria Camargos Rocha; Maria das Graças Pimenta Sanna; Juliana Becattini Guerra; Renato Dani; Maria de Lourdes Abreu Ferrari; Lúcia Porto Fonseca Castro

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 95 KB
• Volume: 15
• Category: Article
• ISSN: 1078-0998
• DOI: 10.1002/ibd.20757

✦ Synopsis

Background:

Polymorphisms in genes linked to the innate and adaptive immune response may be involved in inflammatory bowel disease pathogenesis. Our aim was to investigate associations among IL1B-511, IL1B-31, IL1RN, TNFA-307, TLR-2, TLR-4, IL2-330, NOD2 G908R, NOD2 L1007fsinsC polymorphisms and both Crohn's disease (CD) and ulcerative colitis (UC) in a Brazilian population.

Methods:

We studied 43 patients with CD, 42 with UC, and 541 blood donors. Polymorphisms were evaluated by PCR, PCR-CTPP, or PCR-RFLP. Data were analyzed in multivariate models adjusting for confounding factors.

Results: IL1RN VNTR (P ϭ 0.00, odds ratio [OR] ϭ 2.43, 95% confidence interval [CI] ϭ 1.50 -3.90), as well as TNFA-307 polymorphic allele (P ϭ 0.05, OR ϭ 1.70, 95% CI ϭ 1.00 -2.94) were associated with UC. Both NOD2 mutations (G908R, P ϭ 0.02, OR ϭ 6.83, 95% CI ϭ 1.62-25.45, and L1007fsinsC, P ϭ 0.00, OR ϭ 20.00, 95% CI ϭ 3.21-124.69) were associated with CD.

Conclusions:

Our analyses showed positive associations between proinflammatory polymorphisms at IL1RN and TNFA-307 loci and UC, as well as polymorphisms in the NOD2 gene and CD. These results highlight the importance of different genetic profiles associated with CD and UC.