Immobilization of α-chymotrypsin onto hydrolyzed poly(ethylene)-g-co-hydroxyethyl methacrylate

✍ Scribed by M. Alves Da Silva; M. H. Gil; A. J. Guiomar; C. Martins; J. T. Guthrie

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 471 KB
• Volume: 41
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.1990.070410722

✦ Synopsis

Abstract

α‐Chymotrypsin has been immobilized onto partially hydrolyzed poly(ethylene)‐g‐co‐hydroxyethyl methacrylate (120% graft), using 1‐cyclohexyl‐3‐(2‐morpholinoethyl)‐carbodiimide‐p‐toluene‐sulphonate (CMC) as the activating agent. The influence of the enzyme concentration, the carbodiimide concentration, and the coupling medium on the immobilization reaction was studied. A system with 160 mg of coupled enzyme per gram copolymer, providing 126 mg of active enzyme per gram of copolymer was obtained. The K~M~, V~max~, and the optimum temperature profile and optimum pH value for the free and immobilized α‐chymotrypsin were determined.