Immobilization of trypsin onto “molded” macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) rods and use of the conjugates as bioreactors and for affinity chromatography
✍ Scribed by Miroslav Petro; Frantisek Svec; Jean M. J. Fréchet
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 868 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Ttypsin immobilization onto continuous "molded" rods of porous poly(glycidy1 methacrylate-co-ethylene dimethacrylate) and some applications of the conjugate have been studied. The rods polymerized within a tubular mold (chromatographic column), were treated in situ with ethylenediamine, activated with glutaraldehyde and finally modified with trypsin. The performance of the trypsinmodified rods was evaluated and compared to that of poly(glycidy1 methacrylate-co-ethylene dimethactylate) beads, modified with the same enzyme. Overall the enzyme-modified rods performed substantially better than the corresponding beads. In particular, the performance of the molded supports as enzymatic reactors or as chromatographic media benefits greatly from the enhanced mass transfer that is characteristic of the molded rod at high flow rates. 0 1996