Immobilization of thermostable β-galactosidase on epoxy support and its use for lactose hydrolysis and galactooligosaccharides biosynthesis
✍ Scribed by MarÃn-Navarro, Julia; Talens-Perales, David; Oude-Vrielink, Anneloes; Cañada, Francisco J.; Polaina, Julio
- Book ID
- 121435412
- Publisher
- Springer
- Year
- 2013
- Tongue
- English
- Weight
- 606 KB
- Volume
- 30
- Category
- Article
- ISSN
- 1573-0972
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## Abstract Partially purified lactoses (β‐D‐galactoside galactohydrolase, EC 3.2.1.23) from __Aspergillus niger__, __Ladobacillus helveticus__, and __Saccharomyces lactis__ were immobilized on diazotized porous glass particles (mean pore diameter, 86.5 nm: particle size diameters, 75–125 μm). In a
Milk samples were analyzed for their lactose content using flow injection analysis and incorporating immobilized beta-galactosidase or beta-galactosidase/mutarotase and glucose oxidase/peroxidase bioreactors. These enzymes were immobilized, under mild conditions, on to a 2-fluoro-1-methylpyridinium