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Immobilization of pig liver microsomes

✍ Scribed by Magda Ibrahim; Martine Decolin; Anne-Marie Batt; Edith Dellacherie; Gerard Siest

Book ID
112896212
Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
838 KB
Volume
12
Category
Article
ISSN
0273-2289

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Pur i f ied epox ide hydrolase was immobilized by covalent binding to Sephadex G-150 activated with 1,l' carbonyl diimidazole under mild conditions Kmapp values of free and immobilized epoxide hydrolase were 0.5~ M and 2~ M respectively towards benzo(a)p rene-4,5-oxide,-YherEys Vmaxapp was decreased