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Immobilization of epoxide hydrolase purified from rat liver microsomes

✍ Scribed by Magda Ibrahim; Patrick Hubert; Edith Dellacherie; Jacques Magdalou; Gérard Siest

Book ID
104635602
Publisher
Springer Netherlands
Year
1984
Tongue
English
Weight
261 KB
Volume
6
Category
Article
ISSN
0141-5492

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✦ Synopsis

Pur i f ied epox ide hydrolase was immobilized by covalent binding to Sephadex G-150 activated with 1,l' carbonyl diimidazole under mild conditions Kmapp values of free and immobilized epoxide hydrolase were 0.5~ M and 2~ M respectively towards benzo(a)p rene-4,5-oxide,-YherEys Vmaxapp was decreased from 300 nmol.min-'.mg-Y to 81 nmol.min .mg . Immobilization enhanced stability and allowed repeated use of the enzyme.

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