To determine the effect of hyperosmotic stress on the monoclonal antibody (MAb) production by calciumalginate-immobilized S3H5iy2bA2 hybridoma cells, the osmolalities of medium in the M A b production stage were varied through the addition of NaCI. The specific MAb productivity (9Mab) of immobilized
Immobilization of hybridoma cells with alginate and urethane polymer and improved monoclonal antibody production
β Scribed by Shinji Iijima; Takashi Mano; Masayuki Taniguchi; Takeshi Kobayashi
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 366 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1432-0614
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An attempt has been made to mathematically describe and analyze monoclonal antibody (MAB) productivity of hybridoma cells, with particular emphasis on continuous cultures under unsteady-state conditions. A simple and unstructured general kinetic model that takes account of productivity loss during l
## Abstract The flowβcytometric (FCM) analysis of bivariate DNA/lgG distributions has been conducted to study the cell cycle kinetics and monoclonal antibody (MAb) production during perfusion culture of hybridoma cells. Three different perfusion rates were employed to demonstrate the dependency of